How to get frequency for frame by frame audio analysis?

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hello everyone , I have read a 5 seconds wav file and from the five Seconds I took 0.025 msec
now I want frequency of that 0.025 msec of audio how can I get grequency for that frame , kindly help me for getting particular frequency of audio how can I know that what is the audio frequency ? your answer and guidance will highly appreciated .

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