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You can use the Statistics and Machine Learning Toolbox™ function `anovan`

to perform *N*-way
ANOVA. Use *N*-way ANOVA to determine if the means
in a set of data differ with respect to groups (levels) of multiple
factors. By default, `anovan`

treats all grouping
variables as fixed effects. For an example of ANOVA with random effects,
see ANOVA with Random Effects.
For repeated measures, see `fitrm`

and `ranova`

.

*N*-way ANOVA is a generalization of two-way
ANOVA. For three factors, for example, the model can be written as

$${y}_{ijkr}=\mu +{\alpha}_{i}+{\beta}_{j}+{\gamma}_{k}+{(\alpha \beta )}_{ij}+{(\alpha \gamma )}_{ik}+{(\beta \gamma )}_{jk}+{(\alpha \beta \gamma )}_{ijk}+{\epsilon}_{ijkr,}$$

where

*y*is an observation of the response variable._{ijkr}*i*represents group*i*of factor*A*,*i*= 1, 2, ...,*I*,*j*represents group*j*of factor*B*,*j*= 1, 2, ...,*J*,*k*represents group*k*of factor C, and*r*represents the replication number,*r*= 1, 2, ...,*R*. For constant*R*, there are a total of*N*=*I***J***K***R*observations, but the number of observations does not have to be the same for each combination of groups of factors.*μ*is the overall mean.*α*are the deviations of groups of factor_{i}*A*from the overall mean*μ*due to factor*A*. The values of*α*sum to 0._{i}$${\sum}_{i=1}^{I}{\alpha}_{i}}=0.$$

*β*are the deviations of groups in factor_{j}*B*from the overall mean*μ*due to factor*B*. The values of*β*sum to 0._{j}$${\sum}_{j=1}^{J}{\beta}_{j}}=0.$$

*γ*are the deviations of groups in factor_{k}*C*from the overall mean*μ*due to factor*C*. The values of*γ*sum to 0._{k}$${\sum}_{k=1}^{K}{\gamma}_{k}}=0.$$

(

*αβ*)_{ij}is the interaction term between factors*A*and*B*. (*αβ*)_{ij}sum to 0 over either index.$${\sum}_{i=1}^{I}{\left(\alpha \beta \right)}_{ij}}={\displaystyle {\sum}_{j=1}^{J}{\left(\alpha \beta \right)}_{ij}}=0.$$

(

*αγ*)_{ik}is the interaction term between factors*A*and*C*. The values of (*αγ*)_{ik}sum to 0 over either index.$${\sum}_{i=1}^{I}{\left(\alpha \gamma \right)}_{ik}}={\displaystyle {\sum}_{k=1}^{K}{\left(\alpha \gamma \right)}_{ik}}=0.$$

(

*βγ*)_{jk}is the interaction term between factors*B*and*C*. The values of (*βγ*)_{jk}sum to 0 over either index.$${\sum}_{j=1}^{J}{\left(\beta \gamma \right)}_{jk}}={\displaystyle {\sum}_{k=1}^{K}{\left(\beta \gamma \right)}_{jk}}=0.$$

(

*αβγ*)_{ijk}is the three-way interaction term between factors*A*,*B*, and*C*. The values of (*αβγ*)_{ijk}sum to 0 over any index.$${\sum}_{i=1}^{I}{\left(\alpha \beta \gamma \right)}_{ijk}}={\displaystyle {\sum}_{j=1}^{J}{\left(\alpha \beta \gamma \right)}_{ijk}}={\displaystyle {\sum}_{k=1}^{K}{\left(\alpha \beta \gamma \right)}_{ijk}}=0.$$

*ε*are the random disturbances. They are assumed to be independent, normally distributed, and have constant variance._{ijkr}

Three-way ANOVA tests hypotheses about the effects of factors *A*, *B*, *C*,
and their interactions on the response variable *y*.
The hypotheses about the equality of the mean responses for groups
of factor *A* are

$$\begin{array}{l}{H}_{0}:{\alpha}_{1}={\alpha}_{2}\cdots ={\alpha}_{I}\\ {H}_{1}:\text{atleastone}{\alpha}_{i}\text{isdifferent},\text{}i=1,\text{}2,\text{}\mathrm{...},\text{}I.\end{array}$$

The hypotheses about the equality of the mean response for groups
of factor *B* are

$$\begin{array}{l}{H}_{0}:{\beta}_{1}={\beta}_{2}=\cdots ={\beta}_{J}\\ {H}_{1}:\text{atleastone}{\beta}_{j}\text{isdifferent,}j=1,\text{}2,\text{}\mathrm{...},\text{}J.\end{array}$$

The hypotheses about the equality of the mean response for groups
of factor *C* are

$$\begin{array}{l}{H}_{0}:{\gamma}_{1}={\gamma}_{2}=\cdots ={\gamma}_{K}\\ {H}_{1}:\text{atleastone}{\gamma}_{k}\text{isdifferent},\text{}k=1,\text{}2,\text{}\mathrm{...},\text{}K.\end{array}$$

The hypotheses about the interaction of the factors are

$$\begin{array}{l}{H}_{0}:{\left(\alpha \beta \right)}_{ij}=0\\ {H}_{1}:\text{atleastone}{\left(\alpha \beta \right)}_{ij}\ne 0\end{array}$$

$$\begin{array}{l}{H}_{0}:{\left(\alpha \gamma \right)}_{ik}=0\\ {H}_{1}:\text{atleastone}{\left(\alpha \gamma \right)}_{ik}\ne 0\\ \\ {H}_{0}:{\left(\beta \gamma \right)}_{jk}=0\\ {H}_{1}:\text{atleastone}{\left(\beta \gamma \right)}_{jk}\ne 0\\ \\ {H}_{0}:{\left(\alpha \beta \gamma \right)}_{ijk}=0\\ {H}_{1}:\text{atleastone}{\left(\alpha \beta \gamma \right)}_{ijk}\ne 0\end{array}$$

In this notation parameters with two subscripts, such as (*αβ*)_{ij},
represent the interaction effect of two factors. The parameter (*αβγ*)_{ijk} represents
the three-way interaction. An ANOVA model can have the full set of
parameters or any subset, but conventionally it does not include complex
interaction terms unless it also includes all simpler terms for those
factors. For example, one would generally not include the three-way
interaction without also including all two-way interactions.

Unlike `anova1`

and `anova2`

, `anovan`

does
not expect data in a tabular form. Instead, it expects a vector of
response measurements and a separate vector (or text array) containing
the values corresponding to each factor. This input data format is
more convenient than matrices when there are more than two factors
or when the number of measurements per factor combination is not constant.

$$\begin{array}{ccccccccccc}y& =& [& {y}_{1},& {y}_{2},& {y}_{3},& {y}_{4},& {y}_{5},& \cdots ,& {y}_{N}& {]}^{\prime}\\ & & & \uparrow & \uparrow & \uparrow & \uparrow & \uparrow & & \uparrow & \\ g1& =& \{& \text{'}A\text{'},& \text{'}A\text{'},& \text{'}C\text{'},& \text{'}B\text{'},& \text{'}B\text{'},& \cdots ,& \text{'}D\text{'}& \}\\ g2& =& [& 1& 2& 1& 3& 1& \cdots ,& 2& ]\\ g3& =& \{& \text{'}\text{hi}\text{'},& \text{'}\text{mid}\text{'},& \text{'}\text{low}\text{'},& \text{'}\text{mid}\text{'},& \text{'}\text{hi}\text{'},& \cdots ,& \text{'}\text{low}\text{'}& \}\end{array}$$

This example shows how to perform N-way ANOVA on car data with mileage and other information on 406 cars made between 1970 and 1982.

Load the sample data.

`load carbig`

The example focusses on four variables. `MPG`

is the number of miles per gallon for each of 406 cars (though some have missing values coded as `NaN`

). The other three variables are factors: `cyl4`

(four-cylinder car or not), `org`

(car originated in Europe, Japan, or the USA), and `when`

(car was built early in the period, in the middle of the period, or late in the period).

Fit the full model, requesting up to three-way interactions and Type 3 sums-of-squares.

varnames = {'Origin';'4Cyl';'MfgDate'}; anovan(MPG,{org cyl4 when},3,3,varnames)

`ans = `*7×1*
0.0000
NaN
0.0000
0.7032
0.0001
0.2072
0.6990

Note that many terms are marked by a # symbol as not having full rank, and one of them has zero degrees of freedom and is missing a *p*-value. This can happen when there are missing factor combinations and the model has higher-order terms. In this case, the cross-tabulation below shows that there are no cars made in Europe during the early part of the period with other than four cylinders, as indicated by the 0 in `tbl(2,1,1)`

.

[tbl,chi2,p,factorvals] = crosstab(org,when,cyl4)

tbl = tbl(:,:,1) = 82 75 25 0 4 3 3 3 4 tbl(:,:,2) = 12 22 38 23 26 17 12 25 32

chi2 = 207.7689

p = 8.0973e-38

`factorvals = `*3x3 cell array*
{'USA' } {'Early'} {'Other' }
{'Europe'} {'Mid' } {'Four' }
{'Japan' } {'Late' } {0x0 double}

Consequently it is impossible to estimate the three-way interaction effects, and including the three-way interaction term in the model makes the fit singular.

Using even the limited information available in the ANOVA table, you can see that the three-way interaction has a *p*-value of 0.699, so it is not significant.

Examine only two-way interactions.

[p,tbl2,stats,terms] = anovan(MPG,{org cyl4 when},2,3,varnames);

terms

`terms = `*6×3*
1 0 0
0 1 0
0 0 1
1 1 0
1 0 1
0 1 1

Now all terms are estimable. The *p*-values for interaction term 4 (`Origin*4Cyl`

) and interaction term 6 (`4Cyl*MfgDate`

) are much larger than a typical cutoff value of 0.05, indicating these terms are not significant. You could choose to omit these terms and pool their effects into the error term. The output `terms`

variable returns a matrix of codes, each of which is a bit pattern representing a term.

Omit terms from the model by deleting their entries from `terms`

.

terms([4 6],:) = []

`terms = `*4×3*
1 0 0
0 1 0
0 0 1
1 0 1

Run `anovan`

again, this time supplying the resulting vector as the model argument. Also return the statistics required for multiple comparisons of factors.

[~,~,stats] = anovan(MPG,{org cyl4 when},terms,3,varnames)

`stats = `*struct with fields:*
source: 'anovan'
resid: [1x406 double]
coeffs: [18x1 double]
Rtr: [10x10 double]
rowbasis: [10x18 double]
dfe: 388
mse: 14.1056
nullproject: [18x10 double]
terms: [4x3 double]
nlevels: [3x1 double]
continuous: [0 0 0]
vmeans: [3x1 double]
termcols: [5x1 double]
coeffnames: {18x1 cell}
vars: [18x3 double]
varnames: {3x1 cell}
grpnames: {3x1 cell}
vnested: []
ems: []
denom: []
dfdenom: []
msdenom: []
varest: []
varci: []
txtdenom: []
txtems: []
rtnames: []

Now you have a more parsimonious model indicating that the mileage of these cars seems to be related to all three factors, and that the effect of the manufacturing date depends on where the car was made.

Perform multiple comparisons for Origin and Cylinder.

`results = multcompare(stats,'Dimension',[1,2])`

`results = `*15×6*
1.0000 2.0000 -5.4891 -3.8412 -2.1932 0.0000
1.0000 3.0000 -4.4146 -2.7251 -1.0356 0.0001
1.0000 4.0000 -9.9992 -8.5828 -7.1664 0.0000
1.0000 5.0000 -14.0237 -12.4240 -10.8242 0.0000
1.0000 6.0000 -12.8980 -11.3080 -9.7180 0.0000
2.0000 3.0000 -0.7171 1.1160 2.9492 0.5085
2.0000 4.0000 -7.3655 -4.7417 -2.1179 0.0000
2.0000 5.0000 -9.9992 -8.5828 -7.1664 0.0000
2.0000 6.0000 -9.7464 -7.4668 -5.1872 0.0000
3.0000 4.0000 -8.5396 -5.8577 -3.1757 0.0000
⋮

`anova1`

| `anovan`

| `kruskalwallis`

| `multcompare`