how to identify background taking average from a collection of images

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i am having a problem to separate object from a collection of images. those pictures look quite similar still threshold values differ. so i wanted to identify the background taking average from the collection. for the simplicity i am attaching few photos. thanks

Answers (1)

Image Analyst
Image Analyst on 21 Oct 2015
Can't you just take a blank shot - one with the slide in there but no cells at all?

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