How can I plot a sequence of images into one image using one colorscale as indicator of sequence position (Intensity-over-time)?

Hello,

I want to see an intensity-over-time profile of an image sequence (i.e. the bleaching of a fluorophore over time). I created a 1x300 cell array of images á 128x128 pixel. Each cell resembles a frame of the sequence. Afterwards I subtracted the last frame from all other frames. Now, I want to encode the sequence position with one color to generate a single image with the information when the fluorophore bleached.

In short: I want to plot all images into one merged image, but coded with a color scale in that way, that i see from which sequence number the image came from.

I'm quite a beginner in MatLab, sorry for the missing technical terms. thanks!

Answer 1

[Image Analyst]

Use jet(300) or some colormap to get an array of 300 colors, which is one unique color for each of your frames.

myColorMap = jet(300);

Then for each frame, convert to black and white, multiply by the colors for that frame. Something like

rgbForThisFrame{frameNumber} = cat(3, myColorMap(1)*grayImage, myColorMap(2)*grayImage, myColorMap(3)*grayImage);

Then average all 300 color images together to get the one color image that represents them all.

[Janett Göhring]

thanks a lot, that worked fantastically!

For averaging I used following method:

a=zeros(128,128,3);  
for i=1:300,  
    a=a+rgbForThisFrame{i};
end
a=a/299;