how to read axes image and how to access that image

Dear sir I load that image in axes but i can't get that image in subplots

My code :

function imagebtn_Callback(hObject, eventdata, handles)
[FileName,PathName]=uigetfile('*.*','Select any file');
img=fullfile(PathName,FileName);
axes1=imread(img);
axes(handles.axes1);
image(axes1);
guidata(hObject,handles);

function pushbtn1_Callback(hObject, eventdata, handles)
figure
ax(1) = subplot(1,2,1);
axes1= imread(img);
image(axes1); title('RGB image')

Error : ??? Undefined function or variable 'img'.

Error in ==> loimage>pushbtn1_Callback at 107

axes1= imread(img);

Error in ==> gui_mainfcn at 96

feval(varargin{:});

Error in ==> loimage at 42

gui_mainfcn(gui_State, varargin{:});

Error in ==>

@(hObject,eventdata)loimage('pushbtn1_Callback',hObject,eventdata,guidata(hObject))

??? Error while evaluating uicontrol Callback