.tif files not loading in correctly

Hi, I am loading in .tif files for my code, but the problem is when loading it in the values are changing. i.e. for my images they are 1024x1024 .tif files, and when looking at the pixel info using ImageJ, at (686,674) the value is 1.0759. Loading the image into matlab using the fitsread function and looking at (686,674) gives me 1.11037. Has anyone else experience this issue, I am using this image values to calculate temperature arrays, and as a result my arrays are usually 5-8 Kelvin less than expected, I am not sure why when loading in it is doing this. The file classes are uint16 and I have been converting them to doubles without issue. Here is my code

[images, pathname] = uigetfile( ...

{'*.tif', 'All .tif Files (*.tif)'; ...

'*.*','All Files (*.*)'},...

'Select Wind on file to Load');

handles.Calibration_ratio = importTSP_image(images, pathname);

Calibration_ratio = double(handles.Calibration_ratio.('Run9_cam4_ratio'));

function imgdata = importTSP_image(fileToRead1,pathname)

%IMPORTFILE(FILETOREAD1)

% Imports data from the specified file

% FILETOREAD1: file to read

fileToRead1 = fullfile(pathname,fileToRead1);

% Import the file

rawData1 = importdata(fileToRead1);

[~,name] = fileparts(fileToRead1);

newData1.(matlab.lang.makeValidName(name)) = rawData1;

% Create new variables in the base workspace from those fields.

vars = fieldnames(newData1);

for i = 1:length(vars)

assignin('base', vars{i}, newData1.(vars{i}));

end

imgdata = newData1;

end

[Stephen23]

@Dean: please uplpad a sample data file by clicking the paperclip button. And show us the exact code you are using to import that data into MATLAB.

[Dean]

I can not upload a sample data file, but I uploaded the code I use to import

[DGM]

To upload a TIFF file, you'd have to zip it to work around the limitations of the editor. If you're saying you can't upload it for reasons of confidentiality, then that's an obstacle.

[Dean]

Can't upload it for both

[Image Analyst]

Why not? Are they bigger than 5 MB?

Are the values floating point rather than uint8 or uint16 integers?

[DGM]

Is it possible to at least upload any non-identifying information contained in the metadata (e.g. the output from imfinfo(filename) ? TIFF is one of the most flexible image formats, so it's possible that subtle differences could be caused by misinterpreting the file. I'm not familiar with all the edge cases and specialized uses, so I can't really intuit an explanation for the discrepancy without seeing more clues.

[Dean]

So they are floating point TIFs: here is the imfinfo data, the files are below 5 MB

FileModDate: '20-Jul-2023 15:06:46'

FileSize: 4196028

Format: 'tif'

FormatVersion: []

Width: 1024

Height: 1024

BitDepth: 32

ColorType: 'grayscale'

FormatSignature: [77 77 0 42]

ByteOrder: 'big-endian'

NewSubFileType: 0

BitsPerSample: 32

Compression: 'Uncompressed'

PhotometricInterpretation: 'BlackIsZero'

StripOffsets: 1724

SamplesPerPixel: 1

RowsPerStrip: 1024

StripByteCounts: 4194304

XResolution: []

YResolution: []

ResolutionUnit: 'Inch'

Colormap: []

PlanarConfiguration: 'Chunky'

TileWidth: []

TileLength: []

TileOffsets: []

TileByteCounts: []

Orientation: 1

FillOrder: 1

GrayResponseUnit: 0.0100

MaxSampleValue: 4.2950e+09

MinSampleValue: 0

Thresholding: 1

Offset: 8

ImageDescription: 'ImageJ=1.54f↵min=1.2345000173693373E-21↵max=1.9735080003738403↵'

SampleFormat: 'IEEE floating point'

UnknownTags: [2×1 struct]

50838 246 [12,1458]

50839 254 1x1470 double

[Dean]

Basically the pixel values from the original compared to the array that matlab creates when loading are completely different, but I am not sure how, it seems like the image is shifting or some values are being replaced when loading