How to quantify the object shift between projection images using Matlab?
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Hi, I am tasked with quantifying the shift between these 2D simulated images, by implementing an algorithm (e.g. cross-correlation or centre of mass) using matlab. I have attached the data and script for the images. Any help would be appreciated.


Answers (1)
Image Analyst
on 12 Aug 2023
Or try normalized cross correlation. See attached demo.
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Image Analyst
on 13 Aug 2023
Now that I can see your images, why don't you just threshold and find the centroid of the blob? Do this for each image and see how the centroid shifted. Something like
mask1 = grayImage1 > someThresholdValue;
% Take largest blob only.
mask1 = bwareafilt(mask1, 1);
props1 = regionprop(mask1, 'Centroid');
xy1 = props1.Centroid
% Now for image 2
mask2 = grayImage2 > someThresholdValue;
% Take largest blob only.
mask2 = bwareafilt(mask2, 1);
props2 = regionprop(mask2, 'Centroid');
xy2 = props2.Centroid
% Now compute delta x and y
deltax = xy1(1) - xy2(1)
deltay = xy1(2) - xy2(2)
It's a generic, general purpose demo of how to threshold an image to find blobs, and then measure things about the blobs, and extract certain blobs based on their areas or diameters.
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