how to fix this error on gui?

function varargout = WoundAnalysis(varargin)
% WOUNDANALYSIS MATLAB code for WoundAnalysis.fig
%      WOUNDANALYSIS, by itself, creates a new WOUNDANALYSIS or raises the existing
%      singleton*.
%
%      H = WOUNDANALYSIS returns the handle to a new WOUNDANALYSIS or the handle to
%      the existing singleton*.
%
%      WOUNDANALYSIS('CALLBACK',hObject,eventData,handles,...) calls the local
%      function named CALLBACK in WOUNDANALYSIS.M with the given input arguments.
%
%      WOUNDANALYSIS('Property','Value',...) creates a new WOUNDANALYSIS or raises the
%      existing singleton*.  Starting from the left, property value pairs are
%      applied to the GUI before WoundAnalysis_OpeningFcn gets called.  An
%      unrecognized property name or invalid value makes property application
%      stop.  All inputs are passed to WoundAnalysis_OpeningFcn via varargin.
%
%      *See GUI Options on GUIDE's Tools menu.  Choose "GUI allows only one
%      instance to run (singleton)".
%
% See also: GUIDE, GUIDATA, GUIHANDLES

% Edit the above text to modify the response to help WoundAnalysis

% Last Modified by GUIDE v2.5 02-May-2018 11:44:03

% Begin initialization code - DO NOT EDIT
gui_Singleton = 1;
gui_State = struct('gui_Name',       mfilename, ...
                   'gui_Singleton',  gui_Singleton, ...
                   'gui_OpeningFcn', @WoundAnalysis_OpeningFcn, ...
                   'gui_OutputFcn',  @WoundAnalysis_OutputFcn, ...
                   'gui_LayoutFcn',  [] , ...
                   'gui_Callback',   []);
if nargin && ischar(varargin{1})
    gui_State.gui_Callback = str2func(varargin{1});
end

if nargout
    [varargout{1:nargout}] = gui_mainfcn(gui_State, varargin{:});
else
    gui_mainfcn(gui_State, varargin{:});
end
% End initialization code - DO NOT EDIT

% --- Executes just before WoundAnalysis is made visible.
function WoundAnalysis_OpeningFcn(hObject, eventdata, handles, varargin)
% This function has no output args, see OutputFcn.
% hObject    handle to figure
% eventdata  reserved - to be defined in a future version of MATLAB
% handles    structure with handles and user data (see GUIDATA)
% varargin   command line arguments to WoundAnalysis (see VARARGIN)

% Choose default command line output for WoundAnalysis
handles.output = hObject;

% Update handles structure
guidata(hObject, handles);

% UIWAIT makes WoundAnalysis wait for user response (see UIRESUME)
% uiwait(handles.figure1);

% --- Outputs from this function are returned to the command line.
function varargout = WoundAnalysis_OutputFcn(hObject, eventdata, handles) 
% varargout  cell array for returning output args (see VARARGOUT);
% hObject    handle to figure
% eventdata  reserved - to be defined in a future version of MATLAB
% handles    structure with handles and user data (see GUIDATA)

% Get default command line output from handles structure
varargout{1} = handles.output;

% --- Executes on button press in pushbutton1.
function pushbutton1_Callback(hObject, eventdata, handles)
% hObject    handle to pushbutton1 (see GCBO)
% eventdata  reserved - to be defined in a future version of MATLAB
% handles    structure with handles and user data (see GUIDATA)
[filename, pathname] = uigetfile('C:\Program Files\MATLAB\R2016a\bin\WOUND IMAGES\.jpg','Select file');
W = [pathname,filename];
axes(handles.axes1)
X = imread(W);
imshow(X);
handles.ImgData = X;
guidata(hObject,handles);

% --- Executes on button press in pushbutton2.
function pushbutton2_Callback(hObject, eventdata, handles)
% hObject    handle to pushbutton2 (see GCBO)
% eventdata  reserved - to be defined in a future version of MATLAB
% handles    structure with handles and user data (see GUIDATA)
if isfield(handles,'ImgData')
     XY = handles.ImgData;
s=size(XY);
 y=zeros(s(1),s(2));
 for i=1 :s(1)
    for j=1 :s(2)
         if(XY(i,j)>120 && XY(i,j)<215)
            y(i,j)=0;
        else
            y(i,j)=1;
        end
    end
 end 
axes(handles.axes2)
imshow(y); 
end

handles.ImgData1 = y;
guidata(hObject,handles);

function edit1_Callback(hObject, eventdata, handles)
% hObject    handle to edit1 (see GCBO)
% eventdata  reserved - to be defined in a future version of MATLAB
% handles    structure with handles and user data (see GUIDATA)

% Hints: get(hObject,'String') returns contents of edit1 as text
%        str2double(get(hObject,'String')) returns contents of edit1 as a double

% --- Executes during object creation, after setting all properties.
function edit1_CreateFcn(hObject, ~, ~)
% hObject    handle to edit1 (see GCBO)
% eventdata  reserved - to be defined in a future version of MATLAB
% handles    empty - handles not created until after all CreateFcns called

% Hint: edit controls usually have a white background on Windows.
%       See ISPC and COMPUTER.
if ispc && isequal(get(hObject,'BackgroundColor'), get(0,'defaultUicontrolBackgroundColor'))
    set(hObject,'BackgroundColor','white');
end

function edit2_Callback(hObject, eventdata, handles)
% hObject    handle to edit2 (see GCBO)
% eventdata  reserved - to be defined in a future version of MATLAB
% handles    structure with handles and user data (see GUIDATA)

% Hints: get(hObject,'String') returns contents of edit2 as text
%        str2double(get(hObject,'String')) returns contents of edit2 as a double

% --- Executes during object creation, after setting all properties.
function edit2_CreateFcn(hObject, eventdata, handles)
% hObject    handle to edit2 (see GCBO)
% eventdata  reserved - to be defined in a future version of MATLAB
% handles    empty - handles not created until after all CreateFcns called

% Hint: edit controls usually have a white background on Windows.
%       See ISPC and COMPUTER.
if ispc && isequal(get(hObject,'BackgroundColor'), get(0,'defaultUicontrolBackgroundColor'))
    set(hObject,'BackgroundColor','white');
end

function edit3_Callback(hObject, eventdata, handles)
% hObject    handle to edit3 (see GCBO)
% eventdata  reserved - to be defined in a future version of MATLAB
% handles    structure with handles and user data (see GUIDATA)

% Hints: get(hObject,'String') returns contents of edit3 as text
%        str2double(get(hObject,'String')) returns contents of edit3 as a double

% --- Executes during object creation, after setting all properties.
function edit3_CreateFcn(hObject, eventdata, handles)
% hObject    handle to edit3 (see GCBO)
% eventdata  reserved - to be defined in a future version of MATLAB
% handles    empty - handles not created until after all CreateFcns called

% Hint: edit controls usually have a white background on Windows.
%       See ISPC and COMPUTER.
if ispc && isequal(get(hObject,'BackgroundColor'), get(0,'defaultUicontrolBackgroundColor'))
    set(hObject,'BackgroundColor','white');
end

function edit5_Callback(hObject, eventdata, handles)
% hObject    handle to edit5 (see GCBO)
% eventdata  reserved - to be defined in a future version of MATLAB
% handles    structure with handles and user data (see GUIDATA)

% Hints: get(hObject,'String') returns contents of edit5 as text
%        str2double(get(hObject,'String')) returns contents of edit5 as a double

% --- Executes during object creation, after setting all properties.
function edit5_CreateFcn(hObject, eventdata, handles)
% hObject    handle to edit5 (see GCBO)
% eventdata  reserved - to be defined in a future version of MATLAB
% handles    empty - handles not created until after all CreateFcns called

% Hint: edit controls usually have a white background on Windows.
%       See ISPC and COMPUTER.
if ispc && isequal(get(hObject,'BackgroundColor'), get(0,'defaultUicontrolBackgroundColor'))
    set(hObject,'BackgroundColor','white');
end

function edit6_Callback(hObject, eventdata, handles)
% hObject    handle to edit6 (see GCBO)
% eventdata  reserved - to be defined in a future version of MATLAB
% handles    structure with handles and user data (see GUIDATA)

% Hints: get(hObject,'String') returns contents of edit6 as text
%        str2double(get(hObject,'String')) returns contents of edit6 as a double

% --- Executes during object creation, after setting all properties.
function edit6_CreateFcn(hObject, eventdata, handles)
% hObject    handle to edit6 (see GCBO)
% eventdata  reserved - to be defined in a future version of MATLAB
% handles    empty - handles not created until after all CreateFcns called

% Hint: edit controls usually have a white background on Windows.
%       See ISPC and COMPUTER.
if ispc && isequal(get(hObject,'BackgroundColor'), get(0,'defaultUicontrolBackgroundColor'))
    set(hObject,'BackgroundColor','white');
end

function edit7_Callback(hObject, eventdata, handles)
% hObject    handle to edit7 (see GCBO)
% eventdata  reserved - to be defined in a future version of MATLAB
% handles    structure with handles and user data (see GUIDATA)

% Hints: get(hObject,'String') returns contents of edit7 as text
%        str2double(get(hObject,'String')) returns contents of edit7 as a double

% --- Executes during object creation, after setting all properties.
function edit7_CreateFcn(hObject, eventdata, handles)
% hObject    handle to edit7 (see GCBO)
% eventdata  reserved - to be defined in a future version of MATLAB
% handles    empty - handles not created until after all CreateFcns called

% Hint: edit controls usually have a white background on Windows.
%       See ISPC and COMPUTER.
if ispc && isequal(get(hObject,'BackgroundColor'), get(0,'defaultUicontrolBackgroundColor'))
    set(hObject,'BackgroundColor','white');
end

% --- Executes on button press in pushbutton8.
function pushbutton8_Callback(hObject, eventdata, handles)
% hObject    handle to pushbutton8 (see GCBO)
% eventdata  reserved - to be defined in a future version of MATLAB
% handles    structure with handles and user data (see GUIDATA)
J = handles.ImgData1;

meanval = mean2(J);
stdval  = std2(J);
%V  = skewness(J);
Variance = mean2(var(double(J)));

feat = [meanval, stdval, Variance];

set(handles.edit5,'string',meanval);
set(handles.edit6,'string',stdval);
set(handles.edit7,'string',Variance);

handles.ImgData2 = J;
guidata(hObject,handles);

% --- Executes on button press in pushbutton9.
function pushbutton9_Callback(hObject, eventdata, handles)
% hObject    handle to pushbutton9 (see GCBO)
% eventdata  reserved - to be defined in a future version of MATLAB
% handles    structure with handles and user data (see GUIDATA)
close all

[Hana Laili]

This is the error message i get when trying to run the button in gui

>> WoundAnalysis
Attempt to execute SCRIPT class as a function:
C:\Users\user\Desktop\class.m

Error in ismember>ismemberR2012a (line 140)
    if ~strcmpi(class(a),class(b))

Error in ismember (line 58)
        lia = ismemberR2012a(A,B);

Error in cell/ismember (line 74)
            lia = ismember(icA,indReps);           % Find repeats among original list

Error in matlab.ui.internal.dialog.FileChooser/set.InitialFileName (line 75)
            if any(ismember({'.', '..'}, iFile))

Error in matlab.ui.internal.dialog.FileChooser/initialize (line 359)
            obj.InitialFileName = '';

Error in matlab.ui.internal.dialog.FileOpenChooser/initialize (line 61)
            [e-mail](obj);

Error in matlab.ui.internal.dialog.FileChooser (line 32)
            initialize(obj);

Error in matlab.ui.internal.dialog.FileOpenChooser

Error in uigetputfile_helper (line 33)
    ufd = matlab.ui.internal.dialog.FileOpenChooser();

Error in uigetfile (line 122)
[filename, pathname, filterindex] = uigetputfile_helper(0, varargin{:});

Error in WoundAnalysis>pushbutton1_Callback (line 81)
[filename, pathname] = uigetfile('C:\Program Files\MATLAB\R2016a\bin\WOUND IMAGES\.jpg','Select file');

Error in gui_mainfcn (line 95)
        feval(varargin{:});

Error in WoundAnalysis (line 42)
    gui_mainfcn(gui_State, varargin{:});

Error in matlab.graphics.internal.figfile.FigFile/read>@(hObject,eventdata)WoundAnalysis('pushbutton1_Callback',hObject,eventdata,guidata(hObject))

Error while evaluating UIControl Callback

Answer 1

[Walter Roberson]

You need to remove or rename your C:\Users\user\Desktop\class.m as it is interfering with the important MATLAB function class()

[Shaggy Zee]

What's the solution to error like: Reference to non-extistent field 'ImgData' ?

[Walter Roberson]

pushbutton 8 callback needs to add

if isfield(handles,'ImgData')

[Shaggy Zee]

Its does not work.

Okay at the end of pushbutton 8 I store the image data into the structure like:

handles.ImageData = image_data;

guidata(hObject, handles);

now at the begining of pushbutton 9 I want to use the image data which is stored in the structure here is my syntex:

j = handles.ImageData;

% This is where the error comes in on the above line . Please if you don't mind we can use any platform to talk one on one.

[Walter Roberson]

That is not in evidence in your current code. At the moment you have

function pushbutton8_Callback(hObject, eventdata, handles)
% hObject    handle to pushbutton8 (see GCBO)
% eventdata  reserved - to be defined in a future version of MATLAB
% handles    structure with handles and user data (see GUIDATA)
J = handles.ImgData1;

That only works if pushbutton2_Callback has been run. So you need to use

function pushbutton8_Callback(hObject, eventdata, handles)
% hObject    handle to pushbutton8 (see GCBO)
% eventdata  reserved - to be defined in a future version of MATLAB
% handles    structure with handles and user data (see GUIDATA)
if isfield(handles, 'ImgData1')
  J = handles.ImgData1;
  stuff
end

At the moment your pushbutton9 only does the "close all". If it needs to access some field, it should protect itself with the same kind of if isfield

A better approach would be to use the Enable properties to enable and disable the pushbuttons. For example, pushbutton1_Callback should have something like

enables = {'pushbutton2', 'edit1', 'edit3', 'edit5', 'edit7', 'pushbutton9'};
disables = {'pushbutton8'};
for F = enables
    handles.(F{1}).enable = 'on';
end
for F = disables
    handles.(F{1}).enable = 'off';
end

and

function WoundAnalysis_OpeningFcn(hObject, eventdata, handles, varargin)
enables = {'pushbutton1'};
disables = {'pushbutton2', 'edit1', 'edit3', 'edit5', 'edit7', 'pushbutton8', 'pushbutton9'};
for F = enables
    handles.(F{1}).enable = 'on';
end
for F = disables
    handles.(F{1}).enable = 'off';
end

This way, it is not possible to click on pushbutton8 until pushbutton1 has run and stored ImgData1, so pushbutton8 would not need to check to be sure that imgData1 was present.