IMPROFILE in GUI. Problem to apply it to specific figure outside of GUI.

Hi people,

I have a problem with improfile function. I did GUI and it creates some image picture outside the GUI. I'd like to apply improfile to this picture. What I do I create image, store it like: handles.updated_image. Then I click on "get profile on the current picture" in GUI :in GUI I write something like (improfile(handles.updated_image,x,y) but it doesnt give a profile. I dont really understand how to do this. I'd like to apply it without initial coordinates, like improfile(handles.updated_image).

Thank you.