Can anyone help me?

Can anyone help me with this? I get this error

??? Error using ==> imread at 414 File "C:\Matlab\Colon\15min\4x\M1-E1-I800-G-1.tif" does not exist. Error in ==> dokimi2 at 45 imG=imread(FileNameG,'TIFF');

This is my code:

DirName='C:\Matlab';

if true
Verbose=1;
Organ={'Colon'};
Mag={'4x';'10x'};
TimePoints={'15min'; '30min'; '1hr'; '3hr'; '6hr'; '24hr' };
M=[5;5;5;5;5;4];
S=[8;8;8;8;8;8];
end

if true
  fid=fopen('results.csv','w');
  fprintf(fid,'Organ;Time Point;Mag;Mouse Number;Site;GFP_Total_G;GFP_Total_R;Cy3_Total;Tissue_Pixels\r'); 
end;

if true
  hf=figure;
  ss=get( 0, 'ScreenSize' );
  set(hf,'Position',[100 ss(4)-550 1000 450]); 
end;

if true
load corectimage;
  for o=1:length(Organ)
  disp(sprintf('%s',Organ{o}));
for t=1:length(TimePoints)
  disp(sprintf('%s',TimePoints{t}));
for m=1:length(Mag)
  disp(sprintf('%s',Mag{m}));
for p=1:M(t)
  disp(sprintf('   %d',p));
for q=1:S(t)
end

if true
  if strcmp(Organ,'Colon')
  FileNameG=sprintf('%s\\%s\\%s\\%s\\M%d-E1-I800-G-%d.tif',DirName,Organ{o},TimePoints{t}, Mag{m}, p, q);
  imG=imread(FileNameG,'TIFF');    
  FileNameC=sprintf('%s\\%s\\%s\\%s\\M%d-E1-I800-C-%d.tif',DirName,Organ{o},TimePoints{t}, Mag{m}, p, q);
  imC=imread(FileNameC,'TIFF');
else
  FileNameG=sprintf('%s\\%s\\%s\\M%d-E1-I800-G-%d.tif',DirName,Organ{o},TimePoints{t}, Mag{m}, p, q);
  imG=imread(FileNameG,'TIFF');
  FileNameC=sprintf('%s\\%s\\%s\\M%d-E1-I800-C-%d.tif',DirName,Organ{o},TimePoints{t}, Mag{m}, p, q);
  imC=imread(FileNameC,'TIFF');
end 
end;

% Correct the image intensity (only for Mag=4x)

if true
  if strcmp(Mag,'4x')
  imG=double(imG);
  imC=double(imC);
  load(sprintf('%s\\corectimage.mat',DirName));
  imG(:,:,1)=imG(:,:,1).*imXG;
  imG(:,:,2)=imG(:,:,2).*imXG;
  imG(:,:,3)=imG(:,:,3).*imXG;
  imC(:,:,1)=imC(:,:,1).*imXC;
  imC(:,:,2)=imC(:,:,2).*imXC;
  imC(:,:,3)=imC(:,:,3).*imXC;
  imG=uint8(imG);
  imC=uint8(imC);
end;
end

if true
 if Verbose 
  disp('Creating Histograms ...'); 
  [NG,XG]=imhist(imG(:,:,2),256);
  [NC,XC]=imhist(imC(:,:,2),256);
end;

end

if true
  if Verbose 
  disp('Correcting and Thresholding ...'); 
end;

if true
 imG2=(medfilt2(double(imG(:,:,2)).*imXG,[10 10])); 
ff=0.9;
% Dont have to correct for 10x
if strcmp(Mag{o},'10x') 
  imG2=(medfilt2(double(imG(:,:,2)),[10 10])); 
  ff=0.3;
end;
imG2=imG2-min(min(imG2));
imG2=imG2./max(max(imG2));
se = strel('disk',5);
L=(mean2(imG2)-ff*std2(imG2));
BW=im2bw(imG2,L);
imT=imclose(BW,se);
end

if true
  if Verbose 
  disp('Calulating and displaying ...');
end;
Tissue_Pixels=sum(sum(imT));
GFP_Total_G = sum(sum(imG(:,:,2)));
GFP_Total_R = sum(sum(imG(:,:,1)));
GFP_PerTissue_G = GFP_Total_G/sum(sum(double(imT)));
GFP_PerTissue_R = GFP_Total_R/sum(sum(double(imT)));
Cy3_Total = sum(sum(imC(:,:,2)));
Cy3_PerTissue = Cy3_Total/sum(sum(double(imT)));
end

if true
  if Verbose 
  subplot(2,2,1);
  imshow([imG imC]);
  title('GFP and Cy3 Normalized Fluorescence Images');
  subplot(2,2,2);
  plot(XG,NG,'r');
  hold on;
  plot(XC,NC,'g');
  hold off;
  axis tight;
  title('Histograms of GFP (green) and Cy3 (Red) images');
  subplot(2,2,3);
  imagesc([imG2 imT]);colormap(gray);
  title('Total Fluorescence and Total Tissue Mask');
  ha6 = subplot(2,2,4);
  cla(ha6);
  text(0,1,strcat('GFP File: ',FileNameG));
  text(0,0.85,strcat('Cy3 File: ',FileNameC));
  text(0,0.65,sprintf('Total GFP Fluorescence: %d (G) %d (R)',GFP_Total_G, GFP_Total_R))
  text(0,0.50,sprintf('Total Cy3 Fluorescence: %d',Cy3_Total))
  text(0,0.30,sprintf('Tissue Pixels: %d',Tissue_Pixels))
  text(0,0.15,sprintf('Normalized GFP Fluorescence: %0.5f (G) %0.5f (R)',GFP_PerTissue_G, GFP_PerTissue_R))
  text(0,0,sprintf('Normalized Cy3 Fluorescence: %0.5f',Cy3_PerTissue))
  axis off

    if (~((p==M(t))&&(q==S(t)))) 
        pause; 
    end;
else
    fprintf(fid,'%s;%s;%s;%d;%d;%d;%d;%d;%d\r',Organ{o},TimePoints{t},Mag{m},p,q,GFP_Total_G,GFP_Total_R,Cy3_Total,Tissue_Pixels); 
end;

end; end; end; end; end;

end

if true
  if (~Verbose)
  fclose(fid);
end;
end

[Matt J]

Please use the

toolbar button to highlight and format your code readably. Thanks.

[Cedric]

Did you check whether the file

 C:\Matlab\Colon\15min\4x\M1-E1-I800-G-1.tif

exists or not?